R1 Motif is the Major Actin Binding Domain of TRIOBP-4

Specificity of Binding of the Plectin Actin-binding Domain to 4 Integrin

Plectin is a major component of the cytoskeleton and links the intermediate filament system to hemidesmosomes by binding to the integrin 4 subunit. Previously, a binding site for 4 was mapped on the actin-binding domain (ABD) of plectin and binding of 4 and F-actin to plectin was shown to be mutually exclusive. Here we show that only the ABDs of plectin and dystonin bind to 4, whereas those of ...

Identification and Characterization of the Actin-Binding Motif of Phostensin

Phostensin, a protein phosphatase 1 F-actin cytoskeleton-targeting subunit encoded by KIAA1949, consists of 165 amino acids and caps the pointed ends of actin filaments. Sequence alignment analyses suggest that the C-terminal region of phostensin, spanning residues 129 to 155, contains a consensus actin-binding motif. Here, we have verified the existence of an actin-binding motif in the C-termi...

RNP-1, an RNA-binding motif is conserved in the DNA-binding cold shock domain.

Sequence analysis has shown that there is a short motif of 8 amino acids, corresponding to the RNP-1 motif found in canonical RNA-binding domains, which is common to two families of apparently unrelated proteins. Many RNA-binding proteins contain the RNP-1 and RNP-2 motifs in an RNA-binding domain. The cold shock domain (CSD) family of proteins, which includes several transcription factors whic...

Structural and Functional Characterization of the F-actin Binding Headpiece Domain of Plant Villin 4

Plant actin-binding protein SCAB1 is dimeric actin cross-linker with atypical pleckstrin homology domain.

SCAB1 is a novel plant-specific actin-binding protein that binds, bundles, and stabilizes actin filaments and regulates stomatal movement. Here, we dissected the structure and function of SCAB1 by structural and biochemical approaches. We show that SCAB1 is composed of an actin-binding domain, two coiled-coil (CC) domains, and a fused immunoglobulin and pleckstrin homology (Ig-PH) domain. We de...